1. Hind-II was the first restriction enzyme isolated from…?
a. PPLO
b. Haemophilus influenzae
c. E. Coli
d. Bacteriophage
Answer: b. Haemophilus influenzae
2. Which of the following is not a genetic vector?
a. Plasmid
b. Phage
c. Cosmid
d. Virusoid
Answer: d. Virusoid
3. Name the scientists who generated the first recombinant DNA molecules in 1972?
a. Nathan
b. Paul Berg
c. Watson Crick
d. Boyer
Answer: b. Paul Berg
4. The main technique involved in agricultural biotechnology is known as…?
a. Tissue culture
b. Transformation
c. Plant breeding
d. DNA replication
Answer: a. Tissue culture
5. Mega plasmids are found in…?
a. E. Coli
b. Agrobacterium tumifaciens
c. Pseudomonas
d. Bacillus subtillis
Answer: b. Agrobacterium tumifaciens
6. Major latest area of research in biotechnology is?
a. Tissue culture
b. Recombinant DNA technology
c. Genomics
d. None of these
Answer: c. Genomics
7. Which is not necessary to execute polymerase chain reactions successfully?
a. All four DNA bases
b. Short DNA primers
c. DNA polymerase
d. DNA library
Answer: d. DNA library
Recommended: Biotechnology Online Practice Test
8. Choose the correct statement about the function of somatostatin:
a. Stimulate pituitary synthesis and release gonadotropins
b. Inhibit the release of growth hormone from the pituitary
c. Inhibit the release of gonadotropins from the pituitary
d. Stimulate pituitary and promotes secretion of growth hormo
Answer: b. Inhibit the release of growth hormone from the pituitary
9. It is now possible to breed plants and animals of desired characters through…?
a. Tissue culture
b. Genetic engineering
c. Ikebana technique
d. Chromosome engineering
Answer: b. Genetic engineering
10. Which of the following was the first commercially grown genetically engineered food to be granted a license for human consumption? Hint: Flavr Savr (pronounced as “flavor saver”)
a. Cotton
b. Rice
c. Tomato
d. Potato
Answer: c. Tomato
11. Which of the following is the chemical sequence method?
a. DNA chip sequencing
b. Eeman’s method
c. Sanger’s method
d. Maxam-Gilbert method
Answer: d. Maxam-Gilbert method
12. Which of the following is called as dideoxynucleotide chain termination method?
a. DNA chip sequencing
b. Eeman’s method
c. Sanger sequencing
d. Automated sequencing method
Answer: c. Sanger sequencing
13. How many ddNTPs are used in Sanger’s method?
a. Two
b. Four
c. One
d. Three
Answer: b. Four
14. What is used in automated DNA sequencing?
a. Radio labeled dNTPs are used
b. Radio labeled ddNTPs are used
c. Fluorescently labeled dNTPs are used
d. Fluorescently labeled ddNTPs are used
Answer: c. Fluorescently labeled dNTPs are used
15. Which chemicals are used in Maxam-Gilbert sequencing for cytosine alteration?
a. Formic acid
b. Hydrazine
c. Dimethyl sulfate
d. Piperidine
Answer: b. Hydrazine
16. Who discovered the restriction enzyme (or restriction endonuclease)?
a. Watson and Crick
b. A. Werner, H. Smith and Nathans
c. Alexander Fleming
d. Paul Berg
Answer: b. A. Werner, H. Smith and Nathans
17. Bacteria protect themselves from viruses by fragmenting viral DNA with…?
a. Ligase
b. Endonuclease
c. Exonuclease
d. Gyrase
Answer: b. Endonuclease
18. Klenow Fragment is a mesophilic DNA polymerase derived from…?
a. DNA Ligase
b. DNA polymerase II
c. DNA polymerase I
d. Reverse Transcriptase
Answer: c. DNA polymerase I
19. Choose the correct statement regarding the term “southern blotting”:
a. Comparison of DNA fragments to two sources
b. Transfer of DNA fragments to electrophoretic gel from cellulose membrane
c. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
d. Attachment of probes to DNA fragments
Answer: c. Transfer of DNA fragments from electrophoretic gel to a nitrocellulose sheet
20. The enzyme-linked immunosorbent assay (ELISA) is used in the detection of…?
a. Hay fever
b. Tetanus
c. AIDS
d. Tuberculosis
Answer: c. AIDS
21. Golden rice is a variety rich in…?
a. Vitamin C
b. Biotin
c. Lysine
d. Β-carotene and ferritin
Answer: d. Β-carotene and ferritin
22. The ends of fragments of DNA molecule are sticky due to…?
a. Unpaired bases
b. Endonuclease
c. Calcium ions
d. Free methylation
Answer: a. Unpaired bases
23. Which one of the following is used as a vector for cloning into higher organisms?
a. Retrovirus
b. Baculovirus
c. Salmonella typhimurium
d. Rhizopus nigricans
Answer: a. Retrovirus
24. Why plasmids are used as cloning vectors?
a. Can be multiplied in culture
b. Self-replication in bacterial cells
c. Can be multiplied in laboratories with the help of enzymes
d. Replicate freely outside bacterial cells
Answer: b. Self-replication in bacterial cells
25. When was the Human Genome Project introduced?
a. 2000
b. 1990
c. 1972
d. 1980
Answer: b. 1990
26. Vaccines prepared through recombinant DNA technology are known as…?
a. First generation vaccines
b. Second generation vaccines
c. Third generation vaccines
d. Fourth generation vaccines
Answer: c. Third generation vaccines
27. Genetically modified crops can be produced by…?
a. Cross breeding
b. Micropropagation
c. Recombinant DNA technology
d. Somatic hybridization
Answer: c. Recombinant DNA technology
28. Transgenic plants are produced by using Ti plasmid from the…?
a. E. Coli
b. Agrobacterium tumifaciens
c. Agrobacterium varians
d. Bacteriophages
Answer: b. Agrobacterium tumifaciens
29. RNA interference is useful for…?
a. Cell proliferation
b. Micropropagation
c. Cell differentiation
d. Cell defence
Answer: d. Cell defence
30. Gene therapy was first used in 1992 for the treatment of a four-year-old girl suffering from…?
a. Adenosine deficiency
b. Growth deficiency
c. Adenine deficiency
d. Adenosine deaminase deficiency
Answer: d. Adenosine deaminase deficiency
