Polymerase Chain Reaction Online Practice Test Quiz

1. Who developed the PCR technique?

Milstein

Kohler

Altman

Kary Mullis

2. Which is used in reverse transcriptase Polymerase chain reaction?

RNA as a template to form DNA

DNA as a template to form sDNA

All options

mRNA as a template to form cDNA

3. Which is the first step of PCR?

Annealing

Primer extension

All options are incorrect

Denaturation

4. PCR is a:

DNA amplification technique

DNA degradation technique

All options

DNA sequencing technique

5. Which are the basic requirements of PCR include?

Two oligonucleotide primers

All options

DNA sequence to be amplified

Heat stable DNA polymerase

6. The process of binding of primer to the denatured strand is called:

Annealing

Denaturation

Binding stage

Renaturation

7. Which of the following is an application of PCR?

Site-specific recombination

Site-specific translocation

Site-directed mutagenesis

All options

8. At which temperature does denaturation of DNA double helix takes place?

60°C

54°C

94°C

74°C

9. Which of the following is not the thermostable Polymerase?

Vent Polymerase

Taq Polymerase

Pfu Polymerase

DNA polymerase III

10. At what temperature does denaturation of DNA takes place?

72°C to 74°C

10°C to 20°C

40°C to 55°C

90°C to 98°C

11. At what temperature do annealing of DNA and primer take place?

74°C

42°C

96°C

54°C

12. Which of the following are the uses of Polymerase chain reaction?

Amplify gene of interest

Detect the presence of transgene in an organism

Construct RAPD maps

All options

13. Pfu and vent Polymerase are more efficient than Taq Polymerase because:

Of more efficient Polymerase activity

Of polymer activity

All options are incorrect

Of proofreading activity

14. Thermus aquaticus is a source of:

Taq Polymerase

Primase enzyme

Vent Polymerase

All options are incorrect

15. Which of the following is true for asymmetric PCR?

Used for generating single-stranded copies for RNA

Used for generating double-stranded copies for DNA sequence

Used for generating double-stranded copies for RNA

Used for generating single-stranded copies for DNA sequence

Test Name:	Polymerase Chain Reaction quiz
Subject Category:	Biology
Quiz Type:	Multiple Choice Questions
Total Score / Questions:	15 / 15
Time Allowed:	20 Min
Who Should Practice this Test:	Students, job seekers, or professionals who are preparing for competitive exams, job interviews, or tests.

Polymerase Chain Reaction Online Practice Test Quiz

PCR Polymerase Chain Reaction Online Mock Test Quiz

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